Rat URAT1 (Urate Transporter 1) ELISA Kit | ELK7468

Rat URAT1 (Urate Transporter 1) ELISA Kit | ELK7468 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC22A12; OAT4L; RST; Solute Carrier Family 22 Member 12, Organic Anion/Urate Transporter; Organic anion transporter 4-like; Urate anion exchanger 1; Renal-specific transporter

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.267 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urate Transporter 1 (URAT1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Urate Transporter 1 (URAT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urate Transporter 1 (URAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urate Transporter 1 (URAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.