Rat VCAM1 (Vascular Cell Adhesion Molecule 1) ELISA Kit | ELK2597

Rat VCAM1 (Vascular Cell Adhesion Molecule 1) ELISA Kit | ELK2597 | Gentaur UK, US & Europe Distribution

Alternative Names: CD106; INCAM-100; L1CAM

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.45 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the OD of the samples to the standard curve.