Rat VCP (Valosin Containing Protein) ELISA Kit | ELK6826

Rat VCP (Valosin Containing Protein) ELISA Kit | ELK6826 | Gentaur UK, US & Europe Distribution

Alternative Names: IBMPFD; p97; TERA; Transitional Endoplasmic Reticulum ATPase; 15S Mg (2+) -ATPase p97 subunit

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Valosin Containing Protein (VCP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Valosin Containing Protein (VCP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Valosin Containing Protein (VCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Valosin Containing Protein (VCP) in the samples is then determined by comparing the OD of the samples to the standard curve.