Rat vWF (Von Willebrand Factor) ELISA Kit | ELK7682

Rat vWF (Von Willebrand Factor) ELISA Kit | ELK7682 | Gentaur UK, US & Europe Distribution

Alternative Names: F8VWF; VWD; von Willebrand antigen 2

Species: Rat

Assay Type: Sandwich

Sensitivity: 49 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: plasma

Assay Lenght: 3.5h

Research Area: Signal transduction;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Willebrand Factor (vWF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Von Willebrand Factor (vWF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Willebrand Factor (vWF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor (vWF) in the samples is then determined by comparing the OD of the samples to the standard curve.