Rat XIAP (Inhibitor Of Apoptosis Protein, X-Linked) ELISA Kit | ELK9200

Rat XIAP (Inhibitor Of Apoptosis Protein, X-Linked) ELISA Kit | ELK9200 | Gentaur UK, US & Europe Distribution

Alternative Names: BIRC4; API3; ILP1; MIHA; XLP2; Baculoviral IAP Repeat-Containing 4; E3 ubiquitin-protein ligase XIAP; IAP-like; Inhibitor of apoptosis protein 3; X-linked inhibitor of apoptosis protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Apoptosis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Apoptosis Protein, X-Linked (XIAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Apoptosis Protein, X-Linked (XIAP) in the samples is then determined by comparing the OD of the samples to the standard curve.