RbcL | Rubisco large subunit, form I (chicken) | AS01 017

RbcL | Rubisco large subunit, form I (chicken) | AS01 017 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from all known plant, algal and cyanobacterial RbcL (Rubisco large subunit of Rubisco Form I) sequences, including Arabidopsis thaliana UniProt: O03042, TAIR: AtCg00490, Synechococcus sp. Q3ALL1

Host: Chicken

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format: Liquid

Tested Application: Immunogluorescence (IF), Immunolocalization (IL), ImmunoGold (IG), Western blot (WB)

Related Products: AS03 037 | Anti-RbcL | Rubisco large subunit, form I and form II (50 µl), rabbit antibodiesAS03 037A | Anti-RbcL | Rubisco large subunit, form I and form II (50 µg affinity purified), rabbit antibodiesAS03 037-HRP| Anti-RbcL | Rubisco large subunit, form I and form II (40 µg, HRP-conjugated), rabbit antibodiesAS15 2955 | Anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodiesAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serumAS09 409 | Rubisco quantitation kit AS15 2994 | Rubisco ELISA quantitation kit AS07 218 | Anti-Rubisco | 557 kDa hexadecamer, rabbit antibody to a whole protein, rabbit antibodiesAS07 259 | Anti-RbcS | Rubisco small subunit (SSU), rabbit antibodiesAS07 222 | Anti-RbcS | Rubisco small subunit (SSU) from pea, rabbit antibodiesRecommended secondary antibodies: Goat anti-chicken IgY (H&L), HRPconjugated or Goat anti-chicken IgY (H&L), ALP conjugatedPlant and algal protein extraction buffer

Recommended Dilutions: (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 µg of total cellular protein, (WB)

Molecular weight: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

Confirmed Reactivity: Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.

Predicted Reactivity: Algae, Dicots, Conifers, Liverworts, Mosses, Prochlorophytes, Welwitschia

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794) .Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.For Rubisco quantification using quantitative western blot technique, anti-RbcL antibody, (AS03 037) combined with Rubisco ready to use standard (AS01 017) is recommended.

Background: Rubisco (Ribulose-1, 5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

Reconstitution: N/A

Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: ATCG00490

Category: Global Antibodies

Research Area: Plant Biology, Photosynthesis