Recombinant Cadherin 16 Antibody [CDH16/1532R] | 34-077

Recombinant Cadherin 16 Antibody [CDH16/1532R] | 34-077 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.

Research Area: Signal Transduction

Tested Application: Flow, IF, IHC-P

Application: Flow Cytometry: 0.5-1ug/10e6 cells
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE) : 0.5-1 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein A affinity chromatography

Clonality: Recombinant Monoclonal

Clone: CDH16/1532R

Isotype: IgG, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Cadherin-16, Kidney-specific cadherin, Ksp-cadherin, CDH16

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that CDH16 (kidney-specific cadherin, Cadherin 16) is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, CDH16 is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. CDH16 has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.