Recombinant CD15 Antibody [FUT4/1478R] | 34-075

Recombinant CD15 Antibody [FUT4/1478R] | 34-075 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Purified human neutrophils were used as the immunogen for the recombinant CD15 antibody.

Research Area: Cancer, Cell Cycle, Immunology, Signal Transduction

Tested Application: Flow, IF, IHC-P

Application: Flow Cytometry: 2-4 ug/million cells in 0.1ml
Immunofluorescence: 2-4 ug/ml
Immunohistochemistry (FFPE) : 5-10 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Optimal dilution of the recombinant CD15 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein A affinity

Clonality: Recombinant Monoclonal

Clone: FUT4/1478R

Isotype: IgG, k

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Alpha- (1, 3) -fucosyltransferase 4, 241-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.