Recombinant hSUMO2-AMC | 6413

hSUMO2-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO2. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO2-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

6413 | Recombinant hSUMO2-AMC DataSheet

Biomolecule/Target: N/A

Synonyms: Recombinant hSUMO2-AMC

Alternates names: N/A

Taglines: Pure and sensitive AMC tagged fluorogenic substrate for deSUMOylating enzymes.

NCBI Gene ID #: 1191

NCBI Gene Symbol: TRPM-2

Gene Source: Human

Accession #: P10909

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: 95%

Assay: RP-HPLC

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity:

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 10.619 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in dHO to a working volume of 0.5 mg/ml and let the lyophilized pellet dissolve completely.

Amino acid sequence: N/A