Recombinant Human DLL-1, soluble Protein [HEK 293 cells] | S01-060S/S01-060

Recombinant Human DLL-1, soluble Protein [HEK 293 cells] | S01-060S/S01-060 | Gentaur UK, US & Europe Distribution

Species: Human

Host / biotech: HEK 293 cells

Comment: N/A

Label: N/A

Clone / Antibody feature: N/A

Subcategory: Soluble Receptors

Category: Recombinant Protein

Synonyms: DLL1; DL1; Delta; DELTA1

Isotype: N/A

Application: N/A

Detection Range: Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 μg/ml.

Species Reactivity/Cross reactivity: Human

Antigen: N/A

Description: Human sDLL-1 comprises the extracellular signaling domain of DLL1, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors. It is expressed in the heart and pancreas, and to a lesser extent in various other tissues. DLL-1 functions to specifically activate the Notch-1 and Notch-2 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. DLL-1 suppresses differentiation of hematopoietic progenitor cells into the B-cell lineage while promoting differentiation to T-cell and NK cell precursors. Recombinant human sDLL-1 is a 57-60 kDa glycoprotein containing 522 amino-acid residues.

Purity Confirmation: > 98% by SDS-PAGE & HPLC analyses

Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)

Formulation: lyophilized

Storage Handling Stability: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.

Reconstituation: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Molecular Weight: 57-60 kDa

Lenght (aa): 522

Protein Sequence: SGVFELKLQE FVNKKGLLGN RNCCRGGAGP PPCACRTFFR VCLKHYQASV SPEPPCTYGS AVTPVLGVDS FSLPDGGGAD SAFSNPIRFP FGFTWPGTFS LIIEALHTDS PDDLATENPE RLISRLATQR HLTVGEEWSQ DLHSSGRTDL KYSYRFVCDE HYYGEGCSVF CRPRDDAFGH FTCGERGEKV CNPGWKGPYC TEPICLPGCD EQHGFCDKPG ECKCRVGWQG RYCDECIRYP GCLHGTCQQP WQCNCQEGWG GLFCNQDLNY CTHHKPCKNG ATCTNTGQGS YTCSCRPGYT GATCELGIDE CDPSPCKNGG SCTDLENSYS CTCPPGFYGK ICELSAMTCA DGPCFNGGRC SDSPDGGYSC RCPVGYSGFN CEKKIDYCSS SPCSNGAKCV DLGDAYLCRC QAGFSGRHCD DNVDDCASSP CANGGTCRDG VNDFSCTCPP GYTGRNCSAP VSRCEHAPCH NGATCHERGH RYVCECARGY GGPNCQFLLP ELPPGPAVVD LTEKLEGQGG PF

NCBI Gene ID: 28514