Recombinant Human FRP-4, soluble Protein [CHO cells] | S01-075S/S01-075

Recombinant Human FRP-4, soluble Protein [CHO cells] | S01-075S/S01-075 | Gentaur UK, US & Europe Distribution

Species: Human

Host / biotech: CHO cells

Comment: N/A

Label: N/A

Clone / Antibody feature: N/A

Subcategory: Soluble Receptors

Category: Recombinant Protein

Synonyms: Secreted frizzled-related protein 4, DDC-4 (rat homologue)

Isotype: N/A

Application: N/A

Detection Range: Determined by its ability to decrease alkaline phosphatase activity in CCL-226 cells when treated with 25ng/ml of Murine Wnt-3a.

Species Reactivity/Cross reactivity: Human

Antigen: N/A

Description: Secreted Frizzled-Related Proteins (sFRPs) are a family of glycosylated Wnt antagonists characterized by a conserved cysteine-rich domain that shares homology with the cysteine-rich, extracellular domain Frizzled proteins use for the binding of Wnt proteins and receptors. Lacking the transmembrane and intracellular domains of the Frizzled proteins, sFRPs function as soluble modulators of the Wnt signaling pathway through the direct binding of Wnt proteins to this cysteine-rich domain, and the resultant inhibition of Wnt receptor binding and signaling capabilities. sFRP-4 is widely distributed in a variety of embryonic and adult tissues where it can function as a circulating antiangiogenic factor, a potent proapoptotic factor, an inhibitor of insulin secretion, and a suppressor of both tumor growth and metastatic potential through disruption of the Wnt signaling pathway. Research has demonstrated the existence of a direct correlation between the downregulation and/or absence of circulating sFRP-4 and the progression of several cancer types, including ovarian, endometrial, prostate and lung. Upregulation of circulating sFRP-4 has been linked to the deterioration of glucose metabolism in the case of type 2 diabetes, as well as the suppression of the keratinocyte hyperproliferation and epidermal hyperlasia that are definitive of psoriasis. CHO cell-derived recombinant human sFRP-4 is a glycoprotein of 328 amino acid residues that contains a cysteine-rich, Frizzled-homologous domain. The calculated molecular weight of CHO cell-derived Recombinant Human sFRP-4 is 37.8 kDa; however, due to glycosylation, the protein migrates at an apparent molecular weight of approximately 55 - 65 kDa by SDS-PAGE analysis under reducing conditions.

Purity Confirmation: > 95% by SDS-PAGE & HPLC analyses

Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)

Formulation: lyophilized

Storage Handling Stability: N/A

Reconstituation: N/A

Molecular Weight: 37, 8 kDa

Lenght (aa): 328

Protein Sequence: VRGAPCEAVR IPMCRHMPWN ITRMPNHLHH STQENAILAI EQYEELVDVN CSAVLRFFLC AMYAPICTLE FLHDPIKPCK SVCQRARDDC EPLMKMYNHS WPESLACDEL PVYDRGVCIS PEAIVTDLPE DVKWIDITPD MMVQERPLDV DCKRLSPDRC KCKKVKPTLA TYLSKNYSYV IHAKIKAVQR SGCNEVTTVV DVKEIFKSSS PIPRTQVPLI TNSSCQCPHI LPHQDVLIMC YEWRSRMMLL ENCLVEKWRD QLSKRSIQWE ERLQEQRRTV QDKKKTAGRT SRSNPPKPKG KPPAPKPASP KKNIKTRSAQ KRTNPKRV

NCBI Gene ID: 6424