Recombinant Human IGF1 Protein | RP00996

Product Overview:

Recombinant Human IGF1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence ( Gly 49 - Ala 118) of human IGF1 (Accession #NP_001104755.1) fused with an Fc, 6×His tag at the C-terminus.

Bioactivity:

Measured by its ability to stimulate p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T human embryonic kidney cells.0.01-1ng/mL of Recombinant Human IGF1 can effectively enhance p70 S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation.|Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1ug/mL (100 uL/well) can bind recombinant human IGF1 with a linear range of 30-250ng/mL.|Measured in a cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is typically 7.5-30 ng/mL.

Source:

Human

Shipping Conditions:

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