Recombinant Human IL-27 Protein [HEK 293 cells] | 100-114S/100-114

Recombinant Human IL-27 Protein [HEK 293 cells] | 100-114S/100-114 | Gentaur UK, US & Europe Distribution

Species: Human

Host / biotech: HEK 293 cells

Comment: N/A

Label: N/A

Clone / Antibody feature: N/A

Subcategory: Cytokines & Growth Factors

Category: Recombinant Protein

Synonyms: Interleukin-27, Interleukin-27 subunit alpha, IL-27-A, Interleukin-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI3, p28, Interleukin-30, IL30

Isotype: N/A

Application: N/A

Detection Range: Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.

Species Reactivity/Cross reactivity: Mouse, Human

Antigen: N/A

Description: As a member of the IL-12 family of heterodimeric cytokines that also includes IL-12, IL-23, and IL-35, IL-27 is formed by the association of an IL-27-p28 subunit (also known as IL-30) with the Epstein-Barr Virus (EBV)-induced Gene 3 (EBI3) subunit (also known as IL-27B). Expressed by antigen-presenting cells (APCs) in the early phases of antigen-mediated activation, IL-27 acts as a critical initiator of adaptive immune responses by promoting the rapid clonal expansion of naïve CD4+ T cells, IFN-gamma production, and Th1 polarization. IL-27 elicits its effects through receptor complexes IL-27R (also known as TCCR/WSX-1) and gp130, a receptor shared by IL-6. Mainly expressed in monocytes, endothelial cells, and dendritic cells, IL-27 plays an important role alongside IL-6 in the regulation of inflammation and autoimmunity; directly antagonizing IL-6’s stimulation of CD4+ T cell proliferation and Th17 differentiation. Recombinant Human IL-27 produced from HEK293 cells is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.

Purity Confirmation: > 90% by SDS-PAGE & HPLC analyses

Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)

Formulation: lyophilized

Storage Handling Stability: N/A

Reconstituation: N/A

Molecular Weight: 47.8 kDa

Lenght (aa): 424

Protein Sequence: p28 subunit: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP EBI3 subunit: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRPRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

NCBI Gene ID: 246778/10148