Recombinant Human SIRT 1 | P1675

Recombinant Human SIRT 1 | P1675

Description: SIRT1 is a member of the sirtuin family of proteins, which are homologs to the yeast Sir2 protein. The sirtuin family contains a sirtuin core domain and is grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin silencing factor. It is an NAD dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53, indicating SIRT1 is involved in the regulation of p53 function via deacetylation. SIRT1 has also been shown to regulate the circadian expression of several core clock genes, and may contribute to the regulation of the circadian rhythm.

Biomolecule/Target:

Synonyms: Sirtuin 1, SIR2L1, hSIRT1, EC 2.3.1.286, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1

Alternates names:

Taglines: Protein deacetylase that links transcriptional regulation directly to intracellular energetics

NCBI Gene ID #: 23411

NCBI Gene Symbol: Human

Accession #: Q96EB6

Recombinant:

Source: Insect cells

Purity by SDS-PAGE: ≥95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): > 1,000 µU/mg

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of SIRT1 activity is the amount of enzyme that hydrolyzes the substrate to yield 1.0 µmol of AFC/min when using ’s Sirtuin Activity Assay Kit, Cat. No. K324.

Molecular Weight: 63.0 kDa (aa 193 to 747, calculated with N-terminal His tag)

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description:

Reconstitution Instructions:

Amino acid sequence: Amino acids 193 to 747 with N-terminal His tag

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans