Recombinant Human Wnt-5a Protein [E. coli] | 400-023

Recombinant Human Wnt-5a Protein [E. coli] | 400-023 | Gentaur UK, US & Europe Distribution

Species: Human

Host / biotech: E. coli

Comment: N/A

Label: His-Tag

Clone / Antibody feature: N/A

Subcategory: Cytokines & Growth Factors

Category: Recombinant Protein

Synonyms: Wnt-5a; Wnt5a; Wnt 5a; Wnt

Isotype: N/A

Application: No direct biological data available at the moment but human Wnt5a binds to immobilized human sROR2 in a functional ELISA.

Detection Range: N/A

Species Reactivity/Cross reactivity: Human

Antigen: N/A

Description: Wnt-5a is one of the most highly investigated non-canonical Wnts and has been implicated in almost all aspects of non-canonical Wnt signalling. In terms of cancer development, Wnt-5a has, until recently, lived in the shadow of its better-characterised relatives. This was largely because of its apparent inability to transform cells or signal through the canonical beta-catenin pathway that is so important in cancer, particularly colorectal cancer. Recent work in a wide range of human tumours has pointed to a critical role for Wnt-5a in malignant progression, but there is conflicting evidence whether Wnt-5a has a tumour-promoting or -suppressing role. Emerging evidence suggests that the functions of Wnt-5a can be drastically altered depending on the availability of key receptors. Hence, the presence or absence of these receptors may go some way to explain the conflicting role of Wnt-5a in different cancers.

Purity Confirmation: > 90% by SDS-PAGE & Coomassie stain

Endotoxin: N/A

Formulation: lyophilized

Storage Handling Stability: The lyophilized human Wnt5a, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted human Wnt5a should be stored in working aliquots at -20°C.

Reconstituation: Human Wnt5a should be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.

Molecular Weight: 36.9 kDa

Lenght (aa): 328

Protein Sequence: MIIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCKLEHHHHHH

NCBI Gene ID: 7474