Recombinant Mouse TNF-alpha Protein [E. coli] | M10-041S/M10-041

Recombinant Mouse TNF-alpha Protein [E. coli] | M10-041S/M10-041 | Gentaur UK, US & Europe Distribution

Species: Mouse

Host / biotech: E. coli

Comment: N/A

Label: N/A

Clone / Antibody feature: N/A

Subcategory: Cytokines & Growth Factors

Category: Recombinant Protein

Synonyms: Tnf; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha

Isotype: N/A

Application: N/A

Detection Range: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.1 ng/ml, corresponding to a specific activity of  ≥ 1 x 107 units/mg.

Species Reactivity/Cross reactivity: Monkey, Rat, Mouse, Human, Chicken, Cow

Antigen: N/A

Description: TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-α is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Purity Confirmation: > 98% by SDS-PAGE & HPLC analyses

Endotoxin: < 0.1 ng/µg of protein (<1EU/µg)

Formulation: lyophilized

Storage Handling Stability: N/A

Reconstituation: N/A

Molecular Weight: 17.3 kDa

Lenght (aa): 156

Protein Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

NCBI Gene ID: 21926