Recombinant PDCD1 / PD-1 Antibody [PDCD1/1410R] | 34-063

Recombinant PDCD1 / PD-1 Antibody [PDCD1/1410R] | 34-063 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: A partial length human protein was used as the immunogen for the recombinant PD1 antibody.

Research Area: Immunology, Cancer, Cell Cycle

Tested Application: IHC-P, IF, Flow

Application: Immunohistochemistry (FFPE) : 1-2 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)
Immunofluorescence: 1-2 ug/ml
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
Titering of the recombinant PD1 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein A affinity chromatography

Clonality: Recombinant Monoclonal

Clone: PDCD1/1410R

Isotype: IgG, k

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: PDCD1 (Programmed cell death-1 protein) , also called PD1 and CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD1 is expressed by few B-cells, so may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma.