RELM-beta, human recombinant | 4654

Human RELM (Resistin-like molecule /FIZZ2) is a new member to the family of adipocyte secreted proteins called adipocytokines. This family includes the RELM , RELM and Resistin molecules. Interestingly, RELM and Resistin share similar characteristics such as an additional cysteine residue within the variable N-terminal region and are both homodimeric proteins. However, the RELM is expressed only in the gastrointestinal track; especially the colon, while the Resistin and RELM are secreted exclusively by adipocytes. Currently, the biological function of these proteins, as well as their molecular targets is larGely unknown. Recombinant Human RELM is a disulfide-linked homodimer with a total molecular weight of 11.0 kDa, consisting of 90 amino acid residue chains.

4654 | RELM-beta human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Resistin-like , RELM , Cysteine-rich secreted protein FIZZ2, Colon and small intestine-specific cysteine-rich protein, Cysteine-rich secreted protein A12--like 1, Colon carcinoma-related gene protein, RELM-b, XCP2, HXCP2

Alternates names: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein

Taglines: Colon and small intestine-specific cysteine-rich protein

NCBI Gene ID #: 197

NCBI Gene Symbol: FETUA

Gene Source: Human

Accession #: P02765

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is 100nM.

Biological activity: Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is 100nM.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 45-55 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: A-Chain: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGelF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL B-Chain: TVVQPSVGAA AGPVVPPCPG RIRHFKV