RELM-beta, murine recombinant | 4655

RELM-beta (Resistin-Like Molecule-beta) is a member of a recently identified family of secreted proteins containing a conserved cystein-rich C-terminus. The RELM family consists of resistin (also called FIZZ3), RELM-alfa (FIZZ1), RELM-beta (FIZZ2) and RELM-gamma. Only resisistin and RELM-beta were found in humans whereas all four RELM family members were identified in rodents. Recombinant Human Relm-beta is a non-glycosylated polypeptide chain consisting of 83 amino acids with a molecular weight of 8,893 kDa.

4655 | RELM-beta murine recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: 9030012B21Rik, Fizz2, Relmb, RELMbeta, Xcp3

Alternates names: Fibroblast Growth Factor-5, HBGF-5, Smag-82

Taglines: Colon and small intestine-specific cysteine-rich protein

NCBI Gene ID #: 2250

NCBI Gene Symbol: FGF5

Gene Source: Human

Accession #: P12034

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Biological activity: Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 27.6 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Tris, pH 7.5, and 25 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG