RELM, murine recombinant | 7196

RELM belongs to a unique family of tissue-specific cytokines termed FIZZ (found in inflammatory zone) and RELM. The other three known members of this family; Resistin, RELM, and RELM, are 85-94 amino acid secreted proteins sharing a conserved C-terminal domain characterized by 10 cysteine residues with a unique spacing motif of C-X11-C-X8-C-X-C-X3-C-X10-C-X-C-X-C-X9-C-C. RELM is most closely related to RELM, but is distinctly secreted to bone marrow, spleen, lung, and in peripheral blood granulocytes. The physiological role of RELM may include the promotion or regulation of promyelocytic differentiation, although the specific molecular targets of RELM have not been identified. Recombinant murine RELM is a 9.2 kDa monomeric protein containing 88 amino acid residues.

7196 | RELM murine recombinant DataSheet

Biomolecule/Target: RELM

Synonyms: Resistin-like gamma

Alternates names: Fibroblast Growth Factor-5, HBGF-5, Smag-82

Taglines: Promotes or regulates promyelocytic differentiation

NCBI Gene ID #: 2250

NCBI Gene Symbol: FGF5

Gene Source: Human

Accession #: P12034

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Biological activity: Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 27.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Tris, pH 7.5, and 25 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG