Description
RELMa Antibody (biotin) | 38-148 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Mouse
Homology: N/A
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mRELMα. Murine RELMα specific antibody was purified by affinity chromatography and then biotinylated.
Research Area: Signal Transduction, Growth Factors, Antibody Pairs
Tested Application: E, WB
Application: ELISA:
Sandwich:
To detect mRELMa by sandwich ELISA (using 100 μ/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine RELMa as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mRELMa.
Western Blot:
To detect mRELMa by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRELMa is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: N/A
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Biotin
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: RELMa antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: HIMF, Xcp2, Fizz1, RELMa, Fizz-1, RELMalpha, RELM-alpha, 1810019L16Rik, Himf, Pmng1, Resistin-like alpha, Cysteine-rich secreted protein A12-gamma,
User Note: Centrifuge vial prior to opening.
BACKGROUND: A family of resistin-like molecules (RELMs) has been identified in rodents and humans. RELM alpha belongs to a unique family of tissue-specific cytokines termed FIZZ (found in inflammatory zone) and RELM. The three known members of this family; Resistin, RELM alpha and RELM beta are 85-94 amino acid secreted proteins sharing a conserved C-terminal domain. RELM alpha and Resistin are secreted exclusively by adipocytes while RELM beta is expressed in the epithelium of the colon and small bowel. The RELMs together with resistin comprise a class of tissue-specific signaling molecules. The physiological role and molecular targets of RELM alpha are still unknown.