Renin (Mouse) ELISA Kit - E4729

E4729 | Renin (Mouse) ELISA Kit DataSheet

Renin is a highly specific endopeptidase with only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. 's Renin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse Renin. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for mouse Renin and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of the mouse Renin. The concentration of Mouse Renin in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : REN1, HNFJ2, renin-1

Tag Line : A Sandwich ELISA kit applies for in vitro quantitative determination of mouse Renin concentrations in serum, plasma and other biological fluids.

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma, Serum, Other biological fluids

Species Reactivity : Mouse

Applications :

Features and Benefits : Sensitivity: 0.10 ng/ml, Detection Range: 0.16—10 ng/ml, Specificity: This kit recognizes Mouse Renin in samples. No significant cross-reactivity or interference between Mouse Renin and analogues was observed.