Resistin Antibody | XP-5260

Resistin Antibody | XP-5260 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Mouse

Homology: N/A

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mResistin (murine Resistin) .

Research Area: Chemokines & Cytokines, Signal Transduction, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
To detect mResistin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mResistin.
Sandwich:
To detect mResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Resistin (XP-5260Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mResistin.
Western Blot:
To detect mResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-mResistin specific antibody was purified by affinity chromatography employing immobilized mResistin matrix.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: Resistin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: ADSF, Rstn, Xcp4, Fizz3, Resistin, Adipose tissue-specific secretory factor, ADSF

User Note: Centrifuge vial prior to opening.

BACKGROUND: Resistin is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.