Resistin, human recombinant | 4559

Human Resistin is a new member to the family of adipocyte secreted proteins. The biological functions of Resistin as well as its molecular target is larGely unknown. Whether it promotes or antagonizes insulin action is still unclear. Recombinant Resistin is a 19.5 kDa dimeric protein consisting of two 92 amino acid polypeptide chains.

4559 | Resistin human recombinant DataSheet

Biomolecule/Target: Resistin

Synonyms: Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12--like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.

Alternates names: Fibroblast Growth Factor-6, HBGF-6, HST-2

Taglines: A cysteine-rich protein resulting in high LDL

NCBI Gene ID #: 2251

NCBI Gene Symbol: FGF6

Gene Source: Human

Accession #: P10767

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Biological activity: Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.7 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 7.5, and 50 mM NaCl.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI