Rev-Erbα polyclonal Antibody | GCP108

Rev-Erbα polyclonal Antibody | GCP108 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Pig

Application: WB

Application Range: WB: 1:5000~1:10000

Background: Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins. Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells. In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm. Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock. Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2. Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR) /HDAC3 complex to target genes to repress transcription.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Rev-Erbα polyclonal Antibody detects endogenous levels of Rev-Erbα protein.

Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1

Immunogen: Synthetic peptide, corresponding to Human Rev-Erbα.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A