RFA | Baker's yeast replication factor A | AS07 214

RFA | Baker's yeast replication factor A | AS07 214 | Gentaur UK, US & Europe Distribution

Immunogen: RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa) ; overexpressed in E.coli and purified by chromatography; no affinity tags were added to any of three subunits

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Western blot (WB)

Related Products: N/A

Recommended Dilutions: ChIP, 1 : 20 000 (WB)

Molecular weight: 70 + 30 + 14 kDa

Confirmed Reactivity: Saccharomyces cerevisiae

Predicted Reactivity: Saccharomyces cerevisiae

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Antibody was also successfully used in ChIP application Holstein et al. (2014) .Load of 1 ng of the protein will allow to visualize two subunits of RPA, while load of 5 ng will allow to visualize all three subunits in Western blot technique.

Background: Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: N/A

Research Area: Fungi