RFK Antibody (N-term) [APR32383G]

RFK Antibody (N-term) [APR32383G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This RFK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RFK.

Cellular Localisation: Cytoplasm.

Molecular Weight: 17623

Clone: Polyclonal

Gene Name: RFK

Gene ID: 55312

Function: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF- activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.

Summary: Tissue Location: Detected in brain, placenta and urinary bladder.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB, FC

Dilution: IHC-P--1:100~500 WB--1:1000 FC--1:10~50

Synonyms: Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK