RFT1 Antibody | 26-488

RFT1 Antibody | 26-488 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFT1.

Research Area: Membrane, Cell Cycle, Signal Transduction

Tested Application: E, WB

Application: RFT1 antibody can be used for detection of RFT1 by ELISA at 1:1562500. RFT1 antibody can be used for detection of RFT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Specificiy: N/A

Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 60 kDa

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by peptide affinity chromatography method.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Concentration: batch dependent

Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store RFT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Alternate Name: RFT1, CDG1N

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a¡¡Man (5) GlcNAc (2) -dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5) GlcNAc (2) -PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man (5) GlcNAc (2) -dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man (5) GlcNAc (2) -PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]) .