RFX1 Antibody | 8199

RFX1 Antibody | 8199 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (89%)

Immunogen: RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.
The immunogen is located within the last 50 amino acids of RFX1.

Research Area: Immunology, Autophagy

Tested Application: E, WB, ICC, IF

Application: RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 108 kDa
Observed: 125 kDa

Validation: N/A

Isoform: N/A

Purification: RFX1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: RFX1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: RFX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: RFX, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, enhancer factor C, EFC, trans-acting regulatory factor 1, transcription factor RFX1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1, 2) . RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3) . RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4) . Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5) .