Ribo Virus (CE) | K-2/C

Ribo Virus | K-2/C is available for delivery

Description:

General information: For rapid purification of viral RNA (HCV,HIV,HGV..) from plasma or serum

Target Disease Type: RNA&DNA purification

Specific Application: Spin Column Purification Method

Storage and Shipping : stock

Ribo Virus (CE) K-2/C DataSheet

INTENDED USE

Kit Ribo Virus is designed for the rapid preparation of highly pure viral nucleic acids (e.g. HCV, HIV, HAV, HDV, Enteroviruses, CMV, HBV*) from fluid biological samples e.g. plasma, serum, urine, bone marrow, swabs, liquor.

PRINCIPLE OF ASSAY

With the Ribo Virus, RNA viruses are lysed quickly and efficiently by lysis buffer RAV1 which is a highly concentrated solution of GITC. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the silica membrane in the Ribo Virus columns. Carrier RNA improves binding and recovery of the low-concentrated viral RNA. Contaminations (potential PCR inhibitors) like salts, metabolites and soluble macromolecular cellular components are removed in simple washing steps with ethanolic buffers RAW and finally RAV3. The nucleic acids can be eluted in low salt buffer or water and are ready-for use in subsequent reactions. The prepared nucleic acids are suitable for applications like automated fluorescent DNA sequencing, RT-PCR, or any kind of enzymatic manipulation. The detection limit for certain viruses depends on individual detection procedures e.g. in-house nested (RT-) PCR. We highly recommend the use of internal standards as well as positive and negative controls in order to monitor the purification, amplification and detection processes.