RIG-I Antibody [Alme-1] | 36-102

RIG-I Antibody [Alme-1] | 36-102 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Recombinant human RIG-I (aa 201-713) .

Research Area: Immunology, Innate Immunity

Tested Application: IHC, IP, WB

Application: Immunohistochemistry: (paraffin sections) . Immunoprecipitation: (1:200) . Western blot: (1:1, 000) .

Specificiy: Recognizes human and mouse RIG-I.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: Alme-1

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: RIG-1; Retinoic Acid-inducible Gene 1 Protein; DEAD-box Protein 58; Probable ATP-dependent RNA Helicase DDX58

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.