Rim2 Antibody | 4609

Rim2 Antibody | 4609 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Rim2 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Rim2.
The immunogen is located within amino acids 250 - 300 of Rim2.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: Rim2 antibody can be used for detection of Rim2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of RIM2 are known to exist. This antibody is predicted to have no cross-reactivity to other Rim proteins.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 39, 128, 148, 170 kDa
Observed: 128 kDa

Validation: N/A

Isoform: N/A

Purification: Rim2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Rim2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Rim2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Rim2 Antibody: OBOE, RIM2, RAB3IP3, KIAA0751, Regulating synaptic membrane exocytosis protein 2, Rab-3-interacting molecule 2, RIM 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Rim2 Antibody: Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner.