RIP3 Antibody | 2283

RIP3 Antibody | 2283 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-RIP3 antibody (2283) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of murine RIP3.
The immunogen is located within the last 50 amino acids of RIP3.

Research Area: Apoptosis

Tested Application: E, IF, IHC-P, WB, IP

Application: WB: 0.1-0.5 μg/mL; IHC-P: 5 μg/mL; IF: 20 μg/mL, IP: 20 μg/mL.
Antibody validated: Western Blot in mouse and human samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse and rat samples; Immunoprecipitation in human samples; Immunogold EM in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1202 – A431 Cell Lysate

Positive Control 2: Cat. No. 1282 - 3T3/NIH Cell Lysate

Positive Control 3: Cat. No. 1285- C2C12 Cell Lysate

Positive Control 4: Cat. No. 1212- 3T3/Balb Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 53kD for mouse RIP3 and 57kD for human RIP3
Observed: 53kD for mouse RIP3 and 57kD for human RIP3

Validation:

KO Validation (Figure 7, 9, 13) shows RIP3 expression detected by anti-RIP3 antibodies (2283) was disrupted in multiple tissues of RIP3 KO mice (figure 7, 13) and in RIP3 KO MEF cells (figure 9) .

KD validation (Figure 10) : Anti-RIP3 antibody (2283) specificity was further verified by RIP3 specific knockdown. RIP3 signal in L929 cells transfected with RIP3 shRNA was disrupted in comparison with that in cells transfected with control shRNA.

Regulated expression validation (Figure 7, 11, 13) : RIP3 expression detected by anit-RIP3 antibodies (2283) was up-regulated by treatment of cerulein (figure 7) , LPS (figure 11) or acetaminophen (figure 13) in different tissues or cells.

Overexpression validation (Figure 12) : RIP3 overexpression detected by anit-RIP3 antibodies (2283) was observed in three cell lines stably transfected with RIP3.

Isoform: Mouse RIP3 has one isoform (486aa, 53kD) . Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD) , isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD) . Rat RIP3 also has one isoform (478aa, 52kD) . 2283 can detect can detect isoforms of mouse and rat as well as human isoform 1.

Purification: RIP3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: RIP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.