RIPK2, Active | 7747

RIPK2 (RIP2; RICK) is a death domain-containing protein kinase. Inohara identified cDNAs encoding a predicted 540-amino acid protein RICK, which contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. Inohara also demonstrated that RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway (1). McCarthy found that overexpression of RIP2 signaled both cell death and NF-kappa-B activation (2). Thome reported that RICK specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (3). Chin generated Ripk2-deficient mice and concluded that RIPK2 is implicated in the innate response to pathogens by NOD and TLR-induced cell signaling and mediates cytokine-induced Ifng production in Th1 and NK cells (4). Also Kobayashi demonstrated that RIPK2 is required for signaling through both TLR and NOD protein family members in the innate immune system as well as for appropriate TCR signaling in the adaptive immune response (5).

7747 | RIPK2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: RIPK, receptor-interacting serine-threonine kinase 2

Alternates names: RIPK, receptor-interacting serine-threonine kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, Short name=CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, Tyrosine-protein kinase RIPK2

Taglines: A potent activator of NF-B and inducer of apoptosis

NCBI Gene ID #: 4254

NCBI Gene Symbol: SCF

Gene Source: N/A

Accession #: P21583

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 20 nmol phosphate incorporated into MBP per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is < 2 ng/ml, corresponding to a specific activity of >5 x 10 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 59.0kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute the lyophilized human SCF in sterile HO to a concentration of 0.1-1 mg/ml. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A