ROBO3 Antibody | 42-734

ROBO3 Antibody | 42-734 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Dog

Immunogen: The immunogen for this antibody is: C-RKDGARLKEEEGR

Research Area: Other

Tested Application: E, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Western Blot: Preliminary experiments gave an approx 25kDa band in Human Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 148kDa according to NP_071765.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Flow cytometry: This antibody was successfully used on rheumatoid arthritis synovial fibroblasts (RASF) as described in Denk et al, Arthritis Res Ther. 2010;12 (2) :R45.; PMID: 20298552.

Specificiy: N/A

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: ROBO3, roundabout, axon guidance receptor, homolog 3 (Drosophila) , HGNC:13433, FLJ21044, HGPPS, RBIG1, RIG1, retinoblastoma inhibiting gene 1, roundabout, axon guidance receptor, homolog 3

User Note: N/A

BACKGROUND: N/A