ROCK1 Antibody, KO Validated | 13-417

ROCK1 Antibody, KO Validated | 13-417 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1) .

Research Area: Apoptosis, Cancer, Cell Cycle, Signal Transduction

Tested Application: WB, IHC, IP

Application: WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:50 - 1:100

Specificiy: N/A

Positive Control 1: 293T

Positive Control 2: U-251MG

Positive Control 3: Jurkat

Positive Control 4: BT-474

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 158kDa

Validation: Antibody is Knockout validated.

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.