ROCK2 Antibody | 6297

ROCK2 Antibody | 6297 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Pig: (100%)

Immunogen: ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2.
The immunogen is located within amino acids 920 - 970 of ROCK2.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 153 kDa
Observed: 160 kDa

Validation: N/A

Isoform: N/A

Purification: ROCK2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ROCK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ROCK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ROCK2 Antibody: ROCK-II, KIAA0619, Rho-associated protein kinase 2, Rho kinase 2, ROCK-II

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ROCK2 Antibody: ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.