RPA3 polyclonal Antibody | BS8384

RPA3 polyclonal Antibody | BS8384 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IF

Application Range: WB 1:500 - 1:2000 IF 1:50 - 1:100

Background: The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB that is composed of three subunits, designated RPA 14 kDa (also known as RPA3), RPA 32 kDa and RPA 70 kDa. Together, these subunits play an important role in DNA replication, recombination and repair. RPA is one of the major damage-recognition structures involved in the early stage of nucleotide excision repair and may play a role in telomere maintenance. The binding of human RPA (hRPA) to DNA involves molecular polarity, in which initial hRPA binding occurs on the 5’ side of a ssDNA substrate and then extends in the 3’ direction to create a stably bound hRPA. The RPA 14 kDa subunit localizes to the nucleus and is the smallest component of the RPA complex, functioning with the other subunits to regulate various aspects of DNA metabolism.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: RPA3 polyclonal Antibody detects endogenous levels of RPA3 protein.

Molecular Weight: ~ 14 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Replication protein A 14 kDa subunit; RP-A p14; Replication factor A protein 3; RF-A protein 3; RPA3; REPA3; RPA14

Immunogen: Recombinant full length Human RPA3.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: