RPT2a | Regulatory particle triple-A ATPase subunit 2a | AS19 4262

RPT2a | Regulatory particle triple-A ATPase subunit 2a | AS19 4262 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant, full lenght RPT2a of Arabidopsis thaliana RPT2a protein sequence UniProt: Q9SZD4-1, TAIR: At4g29040 overexpressed in E.coli, purified from a gel piece

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS19 4260 | Anti-PBA1 | 20S Proteasome beta subunit A1, rabbit antibodiesAS19 4258 | PAC1 | 20S Proteasome alpha subunit C1AS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodiesAS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodiescollection of antibodies to protein modifications and enzymes involved in this process

Recommended Dilutions: 1 : 3000 (WB)

Molecular weight: 49, 4 | 50 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica napus, Cajanus cajan, Gossypium hirsutum, Mucuna pruriens, Noccaea caerulescens, Vigna radiata var. radiata Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: This antibody is also recognizing RPT2b protein. Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.For an image of western blot detection, refer to: Smalle et al. (2002) .

Background: RPT2a (Regulatory particle triple-A ATPase subunit 2a) is the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation. Alternative names: 26S proteasome regulatory subunit 4 homolog A Alternative name (s) : 26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, Protein HALTED ROOT, 26S proteasome regulatory subunit 4 homolog A.

Reconstitution: For reconstitution add 50 µl, of sterile water

Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At4g29040

Category: Plant Proteasome

Research Area: Plant Biology