RSPO3 Antibody | 8153

RSPO3 Antibody | 8153 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3.
The immunogen is located within amino acids 200 - 250 of RSPO3.

Research Area: Stem Cell

Tested Application: E, WB, ICC, IF

Application: RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: RSPO3 antibody is human and mouse reactive. At least three isoforms of RSPO3 are known to exist; this antibody will detect all three.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 30 kDa
Observed: 30 kDa

Validation: N/A

Isoform: N/A

Purification: RSPO3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: RSPO3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: RSPO3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: R-spondin-3, CRISTIN1, PWTSR, THSD2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family) /beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis (1, 2) . RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development (3, 4) .