Description
RXR alpha Antibody | 45-139 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen: The immunogen for this antibody is: CQVNSSLTSPTGRGSM
Research Area: Homeostasis
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.8kDa according to NP_002948.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Solomon et al, J Biol Chem. 1998 Jul 10;273 (28) :17573-8; PMID: 9651351. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 cell line for this particular batch.
Specificiy: This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,
User Note: N/A
BACKGROUND: N/A