S6K1/2 | Ribosomal S6 kinase 1/2 | AS12 1855

S6K1/2 | Ribosomal S6 kinase 1/2 | AS12 1855 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins: S6K1 and S6K2.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4. Contains 0.02% sodium azide.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS12 2608 | Anti-TOR | Target of rapamycin, rabbit antibodiesAS13 2664 | Anti-S6K1-2 | Ribosomal-protein S6 kinase homolog 1, 2 - phosphorylated, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 750-1 : 1000 (WB)

Molecular weight: 52, 6 kDa (S6K1) and 53 kDa (S6K2)

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Thelungiella halophilaSpecies of your interest not listed? Contact us

Not reactive in: Setaria viridis, Solanum lycopersicum; Vitis vinifera

Additional Information: Keeping the samples at 4°C all times is of crucial importance, Laucs buffer was used in example below since this is the one which was used routinely by this test laboratory for the work with kinases, Some unspecific bands can be seen, depending upon western blot protocol which is used, Therefore please consider to use a negative control together with your samples

Background: Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf margins and germinating seeds. Alternative names: AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2) .

Reconstitution: N/A

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G08730, AT3G08720

Category: Translation

Research Area: Plant Biology