SAMHD1 Antibody | 8007

SAMHD1 Antibody | 8007 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Anti-SAMHD1 antibody (8007) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human SAMHD1.

Research Area: Innate Immunity

Tested Application: E, WB, IHC, IF

Application: WB: 1 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot, Immunohistochemistry, Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: SAMHD1 antibody is human and mouse reactive.

Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 72kD
Observed: 72 kD

Validation:

KO Validation (Figure 5) shows SAMHD1 expression detected by anti-SAMHD1 antibodies (8007) was disrupted in multiple cells of SAMHD1 KO mice (figure 6) .

Overexpression validation (Figure 2, 6, 7) : SAMHD1 overexpression detected by anit-SAMHD1 antibodies (8007) was observed in normal cell lines stably across all healthy donors.

Regulated expression validation (Figure 8 & 9) : SAMHD1 expression detected by anit-SAMHD1 antibodies (8007) was down-regulated by nucleofection with miR-181b at 48hr (figure 8) , but up-regulated by  treatment of miR-181family inhibitor (figure 9) .

Isoform: Human SAMHD1 has 3 isoforms, including isoform 1 (626aa, 72kD) , isoform 3 (556aa, 64kD) , isoform 4 (591aa, 68kD) . Mouse SAMHD1 has 2 isoforms, including (658aa, 76kD) and (651aa, 75kD) . Rat SAMHD1 has only one isoform (620aa, 72kD) . 8007 can detect human, mouse and rat.

Purification: SAMHD1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SAMHD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1) . SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2) , and this restriction is regulated by phosphorylation of SAMHD1 (3) . Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1) .