Sar1 | Secretion-associated and Ras-related protein 1 | AS08 326

Sar1 | Secretion-associated and Ras-related protein 1 | AS08 326 | Gentaur UK, US & Europe Distribution

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana UniProt: Q8VYP7, TAIR: At3g62560

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Immunogold (IG), Western blot (WB)

Related Products: AS08 327 | Anti-Sec21p | gamma subunit, COP vesicles, Golgi marker, rabbit antibodiesAS08 325 | Anti-Arf1 | ADP-ribosylation factor 1 rabbit antibodiesSecondary antibodies

Recommended Dilutions: 1 : 50 (IG), 1 : 500 (WB)

Molecular weight: 21 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis, Pteris vittata (fern), Panicum miliaceum, Panicum maximum, Petunia hybrida, Triticum aestivum, Zea mays

Predicted Reactivity: Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Populus trichocarpa, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us

Not reactive in: Nicotiana tabacum

Additional Information: Immunolocalization method with Sar1 antibodies is described in: Yao-dong Yang (2005) . Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005 May;17 (5) :1513-31. Epub 2005 Apr 1.

Background: Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G62560

Category: Endomembrane system

Research Area: Cell Biology