Description
SARS Coronavirus (HSZ-Cc) Nucleocapsid Antibody | 20-271 | Gentaur UK, US & Europe Distribution
Host: Sheep
Reactivity: Virus
Homology: N/A
Immunogen: GST-SARS CoV HSZ-Cc N Protein
Research Area: Infectious Disease, COVID-19
Tested Application: WB
Application: Detection Antibodies: 1 μg/ml Ab, 5% BSA in TBS-T, 4˚C overnight
Blocking: 5% milk in TBS-T, 1h room temp
Secondary Antibodies: 1:15, 000 donkey anti-sheep, 1:15, 000 donkey anti-rabbot, in TBS-T, 1h room temp
Wash: TBS-T, 4x 10 min
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Antibodies are affinity purified against the antigen and eluted with low pH.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Antibody supplied in PBS.
Concentration: batch dependent
Storage Condition: Aliquot and store at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SARS Coronavirus (HSZ-Cc) Nucleocapsid Protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 217, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.