SARS-CoV-2 (COVID-19) Envelope Antibody | 20-274

SARS-CoV-2 (COVID-19) Envelope Antibody | 20-274 | Gentaur UK, US & Europe Distribution

Host: Sheep

Reactivity: Virus

Homology: N/A

Immunogen: GST-SARS-CoV2 E Protein

Research Area: Infectious Disease, COVID-19

Tested Application: WB

Application: Detection Antibodies: 1 μg/ml Ab, 5% BSA in TBS-T, 4˚C overnight
Blocking: 5% milk in TBS-T, 1h room temp
Secondary Antibodies: 1:15, 000 donkey anti-sheep, 1:15, 000 donkey anti-mouse, in TBS-T, 1h room temp
Wash: TBS-T, 4x 10 min

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Antibodies are affinity purified against the antigen and eluted with low pH.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Antibody supplied in PBS.

Concentration: batch dependent

Storage Condition: Aliquot and store at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SARS-CoV-2-E Protein, SARS-CoV-2-Envelope Protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Coronavirus envelope (E) proteins are short (1 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2-3 juxtamembrane cysteines. These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation. In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions, plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction.