SCARB1 Antibody | 5193

SCARB1 Antibody | 5193 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (93%) , Bovine: (80%)

Immunogen: SCARB1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SCARB1.
The immunogen is located within amino acids 70 - 120 of SCARB1.

Research Area: Infectious Disease

Tested Application: E, WB, IHC-P, IF

Application: SCARB1 antibody can be used for detection of SCARB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of SCARB1 are known to exist; this antibody can detect both isoforms.

Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 56, 60 kDa
Observed: 60 kDa

Validation: N/A

Isoform: N/A

Purification: SCARB1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SCARB1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SCARB1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: SCARB1 Antibody: CLA1, SRB1, CLA-1, SR-BI, CD36L1, HDLQTL6, CLA1, Scavenger receptor class B member 1, CD36 and LIMPII analogous 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SCARB1 Antibody: Scavenger receptor class B member 1 (SCARB1) , also known as SR-BI, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARB1 is a multi-ligand cell-surface receptor that mediates the selective uptake of lipid from HDL cholesterol into cells and is expressed in steroidogenic tissues in adult animals. Other ligands of SCARB1 include native, acetylated, or oxidized LDL and anionic phospholipids. SCARB1-deficient mice have elevated HDL levels and increased susceptibility to atherosclerosis on fat feeding, indicating its importance in the regulation of cholesterol homeostasis. Along with CLDN1, LDL-R, and the tetraspanin superfamily member CD81, SCARB1 has been reported to be an entry factor for the Hepatitis C virus.