SCGF-α, human recombinant | 7197

SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-α is a 33.9 kDa polypeptide containing 305 amino acid residues.

7197 | SCGF-α, human recombinant DataSheet

Biomolecule/Target :SCGF-α

Synonyms: Stem Cell Growth Factor-alpha

Alternates names: Stem Cell Growth Factor-alpha

Taglines: Supports early stages of hematopoiesis

NCBI Gene ID #: 4254

NCBI Gene Symbol: SCF

Gene Source: Human

Accession #: P21583

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGE: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug).

Activity (Specifications/test method): Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.

Biological activity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 2.5 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 33.9 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: GHGARGAERE WEGGWGGAQE EEREREALML KHLQEALGLP AGRGDENPAG TVEGKEDWEM EEDQGEEEEE EATPTPSSGP SPSPTPEDIV TYILGRLAGL DAGLHQLHVR LHALDTRVVE LTQGLRQLRN AAGDTRDAVQ ALQEAQGRAE REHGRLEGCL KGLRLGHKCF LLSRDFEAQA AAQARCTARG GSLAQPADRQ QMEALTRYLR AALAPYNWPV WLGVHDRRAE GLYLFENGQR VSFFAWHRSP RPELGAQPSA SPHPLSPDQP NGGTLENCVA QASDDGSWWD HDCQRRLYYV CEFPF

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans