SCGF-, human recombinant | 7198

SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF- is a 25.0 kDa polypeptide containing 227 amino acid residues.

7198 | SCGF- human recombinant DataSheet

Biomolecule/Target: SCGF-

Synonyms: Stem Cell Growth Factor-beta

Alternates names: Glial-Derived Neurotrophic Factor, ATF-1

Taglines: Supports early stages of hematopoiesis

NCBI Gene ID #: 25453

NCBI Gene Symbol: GDNF

Gene Source: Murine

Accession #: Q07731

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of rat C6 cells.

Biological activity: Determined by its ability to stimulate the proliferation of rat C6 cells.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium citrate.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI