Description
SCRAPPER Antibody | 4449 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen: SCRAPPER antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human SCRAPPER.
The immunogen is located within the last 50 amino acids of SCRAPPER.
Research Area: Neuroscience
Tested Application: E, WB, IHC-P
Application: SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1288 - A20 Cell Lysate
Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: SCRAPPER Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SCRAPPER antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: SCRAPPER Antibody: Fbl2, Fbl20, FBL2, F-box and leucine-rich repeat protein 20
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.